3-morpholinopropanesulfonic acid hemisodium salt CAS:117961-20-3

3-(N-Morpholino)propanesulfonic acid hemisodium salt, also known as MOPS-Na, is a zwitterionic buffer commonly used in biochemical and biological research. It is composed of a morpholine ring, a propane chain, and a sulfonic acid group. MOPS-Na is an effective buffer for maintaining a stable pH in the physiological range (pH 6.5-7.9). It is often used in cell culture media, protein purification and characterization, enzyme assays, and DNA/RNA electrophoresis. One of the advantages of MOPS-Na as a buffer is its low UV absorption, which makes it suitable for spectrophotometric applications. It also exhibits minimal interference with common assay methods. MOPS-Na is soluble in water, and its solubility is pH-dependent. It is typically supplied as a solid powder or as a solution, with the hemisodium salt form being more commonly used.

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Application and Effect

pH buffering: MOPS-Na is effective at maintaining a stable pH in the physiological range (pH 6.5-7.9). Its zwitterionic nature allows it to resist changes in pH when acids or bases are added, making it useful for maintaining optimal conditions in cell culture media and various experimental systems. Protein and enzyme studies: MOPS-Na is often used in protein purification, characterization, and stabilization. Its buffering capacity and compatibility with many enzymes and proteins make it suitable for maintaining the desired pH during these processes. MOPS-Na can also be employed in enzyme assays, where precise pH control is essential for accurate measurement of enzymatic activity. DNA and RNA electrophoresis: MOPS-Na is commonly used as a buffer in nucleic acid gel electrophoresis. It provides the desired pH range and ionic strength, allowing for efficient separation of DNA and RNA fragments. The low UV absorption of MOPS-Na is advantageous in this application, as it does not interfere with spectrophotometric measurements of nucleic acids. Cell culture media: MOPS-Na is utilized in cell culture media to maintain the pH and osmotic balance necessary for cell growth and viability. Its compatibility with various cell types and non-toxic nature make it an ideal buffering agent for maintaining physiological conditions in cell culture experiments. Pharmacological and biological research: MOPS-Na is employed in various pharmacological and biological studies, such as measurement of enzyme kinetics, drug screening assays, and studies involving the effects of pH on cellular processes. Its buffering capacity helps to minimize pH-dependent artifacts and ensure reliable and reproducible results.  

Product Packing:

Additional Information:

Composition C7H16NNaO4S
Assay 99%
Appearance White powder
CAS No. 117961-20-3
Packing Small and bulk
Shelf Life 2 years
Storage Store in cool and dry area
Certification ISO.

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